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KMID : 1094720090140040443
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 4 p.443 ~ p.449
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Polyaniline Based Catalase Biosensor for the Detection of Hydrogen Peroxide and Azide
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Singh Ravindra P.
Kang Da-Yeon
Oh Byung-Keun
Choi Jeong-Woo
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Abstract
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The CAT/PANi/ITO bioelectrode has been prepared as a catalase biosensor and shows response for monitoring not only of H2O2 but also azide. The sensor exhibited an excellent response to the H2O2 and azide. The linear range of H2O2 was 0.064~1 mM and for azide 0.125~4 mM, respectively. Catalase biosensor was based on the principle of the measurements as the decrease in the differentiation of oxygen level, which has been caused by the inhibition of catalase in the bioactive layer of the biosensor by azide. The repeatability experiments were done in triplicate. The logarithm response of the biosensor to H2O2 (r2 = 0.99), as well as, for azide (r2 = 0.90) were reported, respectively. The bioelectrode was characterized by CV and AFM. The proposed biosensor would be applied for the determination of H2O2 and azide in various biological samples.
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KEYWORD
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Polyaniline (PANi), catalase (CAT), hydrogen peroxide (H2O2), azide, biosensor, bioelectrode
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